Abdul Rahman S

• Experience in Covid 19 samples (more than 50000 samples) and also experience in RT-PCR diagnosis and reporting. Ensured accurate and timely diagnosis of Covid 19 cases through extensive sample handling and analysis.
• Extraction by using ZYBIO EXM3000- nucleic acid isolation of multiple sample and also have experience in Manual Extraction by using enzymes and buffers. Performed efficient and precise nucleic acid extraction using advanced laboratory techniques, ensuring reliable results.
• Performing analysis, trouble shooting and research interpretation of COVID-19 PCR. Conducted in-depth analysis and troubleshooting of Covid 19 PCR tests, providing valuable insights for research and diagnostic purposes.
• DNA & RNA Extraction by QIAGEN-QIACube, MagGenome Automated Nucleic acid Extraction. Utilized cutting-edge technology for DNA & RNA extraction, improving efficiency and accuracy of the process.
• Performed Inter laboratory quality control (ILQC for Couid-19 samples for fortnight basis, cleared) Internal and External auditing (NABL) for technical testing. Ensured high quality and compliance with industry standards through rigorous quality control measures and internal and external audits.
• Processing External quality assurance service (EQAS) for viral panels. Managed the processing and analysis of external quality assurance service for viral panels, ensuring accurate and reliable results.

• Trained in General Department Procedure for responsible laboratory operations.
• Improved laboratory efficiency and accuracy by implementing standard operating procedures.
• Developed Standard Operation Procedures (SOP) for COVID-19 testing.
• Enhanced laboratory safety and compliance by creating detailed protocols for handling and testing COVID-19 samples.
• Created SOPs for new arrivals of kits and reagents.
• Streamlined laboratory operations and reduced errors by developing standardized procedures for handling new laboratory materials.
• Collaborated with a team of molecular technicians to ensure smooth laboratory operations.
• Promoted teamwork and coordination to maintain efficient and productive laboratory workflow.
• Assisted in conducting molecular biology experiments and data analysis.
• Contributed to groundbreaking research by actively participating in experimental design and data interpretation.

• Demonstrated expertise in performing immunochromatographic tests for various diseases, including Dengue, Widal, Syphilis, and TPHA, contributing to the early detection and treatment of these conditions.
• Utilized advanced laboratory equipment such as Biorad CFX96, Biorad PR4100, and Genexpert for accurate and efficient testing and analysis.
• Employed PCR techniques, including Fluorescent Probe Method, Qualitative detection Method-QUANTIPLUS, and Nucleoprotein (N) and RdRP method HIMEDIA, for sensitive and specific detection of pathogens.
• Successfully screened thousands of patient samples using the ADAGIO system, ensuring the diagnosis of antibacterial activity and guiding appropriate treatment decisions.

MOLECULAR BIOLOGY

Isolation of DNA & RNA from various Clinical specimens (Covid-19,Blood, Urine,BAL, Sputum, Throat swab)

EXTRACTION- ZYBIO-Extraction procedures, Manual Extraction, QIAGEN –for DNA extraction.

PCR - ReverseTranscription PCR, Quantitative PCR.

GENE XPERT- for Tuberculosis.

QIAGEN-For DNA extraction and

experience in the QIAGEN for HCV

MICROBIOLOGY & SEROLOGY

Sample Culture Techniques – Isolation

and culture of all different Clinical specimens of patients such as Urine, Sputum, Pus, Respiratory track

swabs, Tissue, Blood, UTI swabs, All fluids like pleural fluid, Endometrial fluids, Bal fluid and also catheter tip sample

, Nail and Skin crapping etc.

Maintenance Culture – The

patient sample are culture and Incubated via Incubators and observed the Growth of the 24 hrs and 48 hrs incubations.

Query Check – To check the

Growth of the plates of the previous day's samples weather what type of the organism grown in culture by testing

the oxidase and catalase and check by the morphology and reported to give the further followings

AST (Antibiotic Sensitivity Test) – Following the query details do follow the AST drug paternto the selective organism such as Gram positive/Negative, Salmonella, Candida like etc Report and Resulting- ADAGIO –AST readings Report via CLSI guideline.

TRANSPLANT IMMUNOLOGY

Complement Dependent Cytotoxicity Crossmatch (CDCXM): A test that detects if the recipient has antibodies that can react with the donor's cells, using complement activation to assess potential rejection risk.

Flow Crossmatch (FCXM): A modern test that uses flow cytometry to identify antibodies in the recipient's blood that may react with the donor's cells, helping assess transplant compatibility.

Donor Specific Antibody Crossmatch: Tests for antibodies specific to the donor's tissue, which can indicate an increased risk of transplant rejection if present.

Single Antigen Bead Assay (SAB): A test that identifies specific antibodies in the recipient's blood that may react with antigens on the donor's cells, aiding in risk assessment for organ rejection.

Panel Reactive Antibody (PRA): Measures the percentage of a panel of potential donor cells that a recipient’s antibodies can react with, indicating the likelihood of rejection based on antibody presence.

Transplant immunology tests assess how a recipient's immune system responds to a transplanted organ or tissue. These tests help determine the compatibility between donor and recipient to prevent rejection and optimize transplant success.

Transplant Immunology Testing Experience:

• Proficient in performing Complement Dependent Cytotoxicity Crossmatch (CDCXM) and Flow Crossmatch (FCXM) to assess recipient-donor compatibility and rejection risk.
• Experienced with Donor Specific Antibody Crossmatch and Single Antigen Bead Assay (SAB) to identify antibodies and assess transplant success likelihood.
• Skilled in Panel Reactive Antibody (PRA) testing to evaluate recipient antibody reactivity and optimize donor matching.

• Conducted a study to explore the interaction between soil protist Dictyostelium discoideum and rhizospheric bacteria found in Oryza sativa.
• Impact: Contributed to the better implementation of Plant Growth Promoting Rhizobacteria (PGPRs) in agricultural soil.
• Investigated the relationship between bacteria and protist in the context of the soil ecosystem.
• Impact: Provided insights into optimizing agricultural practices for improved crop growth and yield.
• Analyzed the impact of rhizospheric bacteria on the growth and development of Dictyostelium discoideum.
• Impact: Enhanced understanding of the role of microorganisms in plant health and soil fertility.
• Collaborated with a team of researchers to design and execute experiments on the interaction between soil protist and rhizospheric bacteria.
• Impact: Facilitated the development of effective strategies for harnessing the potential of microbial communities in agriculture.
• Published research findings in a peer-reviewed journal, contributing to the growing body of knowledge in the field of soil microbiology.
• Impact: Disseminated valuable information to the scientific community, informing future research and applications in sustainable agriculture.
• Mention measurable impact or results you helped deliver. Focus on the outcome of tasks. Example: Modified customer service support system to reduce complaints by 10%.